Virus:Article Title: A triple-action PROTAC for wild-type p53 cancer therapy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies His-Tag Cell Signaling Technology Cat# 2365S; RRID: AB_2115720 FLAG-Tag Cell Signaling Technology Cat# 2368S; RRID: AB_2217020 BRD4 Cell Signaling Technology Cat# 13440S; RRID: AB_2687578 p53 (DO-1) (Pantropic) Millipore Cat# OP43-100UG; RRID: AB_10682938 p21 Waf1/Cip1 (12S1) Cell Signaling Technology Cat# 2947S; RRID: AB_823586 β-actin Sigma Cat# A1978; RRID: AB_476692 HDM2 (Ab-1) (IF2) Millipore Cat# OP46-100UG; RRID: AB_564803 HDMX/MDM4 Fortis Life Sciences Cat# A300-287A; RRID: AB_263407 Goat anti-Mouse IgG - HRP BioRad Cat# 1706516; RRID: AB_2921252 Goat anti-Rabbit IgG - HRP Cell Signaling Technology Cat# 7074; RRID: AB_2099233 Goat anti-Rabbit IgG - Poly-HRP Invitrogen Cat# 32260; RRID: AB_1965959 BRD2 (D89B4) Cell Signaling Technology Cat# 5848; RRID: AB_10835146 BRD3 (E3D5N) Cell Signaling Technology Cat# 94302 Bacterial and virus strains pET28a vector Addgene Cat# 69864-3 pET15b vector Novagen Cat# 69661 BL21(DE3) E. coli NEB Cat# C2527H pNIC28Bsa4 vector Addgene Cat# 26103 Biological samples Pediatric Leukemia Blood (Primary T-ALL) DFCI Institutional Review Board Protocol #06-078 Chemicals, peptides, and recombinant proteins Ficoll-Paque PREMIUM Fisher Scientific Cat# 45-001-751 RPMI-1640 Gibco Cat# 21780-076 EMEM Gibco Cat# 11095080 DMEM Gibco Cat# 11965118 MEM Gibco Cat# 11095098 McCoy’s 5A (Modified) Medium Gibco Cat# 16600082 Ham’s F-12K (Kaighn’s) Medium Gibco Cat# 2127022 Fetal Bovine Serum Gemini Bio Cat# 100-106 Trypan Blue Gibco Cat# 15250-076 Pen-Strep Gibco Cat# 18140-122 (+)-JQ1 MedChemExpress Cat# HY-13030 (+)-JQ1-carboxylic acid MedChemExpress Cat# HY-78695 (Continued on next page) e1 Cell Reports Medicine 6, 102467, December 16, 2025 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER RG7388 MedChemExpress Cat# HY-15676 A1874 Selleckchem Cat# S8785 dBET6 MedChemExpress Cat# HY-112588 DMSO Sigma Cat# D2650-100ML 1M HEPES, pH 7.5 Gibco Cat# 15630-080 TCEP Thermo Cat# 77720 RIPA lysis buffer Sigma Cat# 20-188 NaCl Fisher Scientific Cat# BP358-212 Human GST-HDM2 R&D Systems Cat# E3-202-050 UBE1 R&D Systems Cat# E− 305-025 UbcH5c/UBE2D3 R&D Systems Cat# E2-627-100 Mg-ATP R&D Systems Cat# B-20 FLAG-p53 BPS Bioscience Cat# 100412 6xHis-BRD4 (BD1+BD2) BPS Bioscience Cat# 31045 4-12% Bis-Tris Gels Invitrogen Cat# NP0335BOX 3-8% Tris-Acetate Gels Invitrogen Cat# EA0375BOX NuPAGE LDS Sample Buffer (4×) Thermo Cat# NP0008 Glycerol Sigma Cat# G9012-1L DTT Gold Biotechnology Cat# 27565-41-9 Isopropyl β-D-1-thiogalactopyranoside Gold Biotechnology Cat# 367-93-1 Protease inhibitor cocktail Sigma Cat# 11873580001 PhosSTOP phosphatase inhibitor Sigma Cat# 4906845001 Urea Sigma Cat# U53758 Iodoacetamide Pierce Cat# A39271 Chloroform Sigma Cat# CX1055 Methanol Fisher Scientific Cat# A412-4 LysC Wako Cat# 129-02541 Trypsin Pierce Cat# 90305 Hydroxylamine Sigma Cat# 467804 TMTpro 18-plex reagents Thermo Cat# A52045 Sep-Pal C18 Waters Cat# WAT020515 Imidazole Sigma Cat# I202 BME Thermo Cat# 21985023 IGEPAL Thermo Cat# J61055.AE PMSF Thermo Cat# 36978 Ni-NTA beads Qiagen Cat# 30210 Bortezomib Sigma Cat# 5043140001 Cycloheximide Thermo Cat# J66004.XF Dynabeads Epoxy 270 Thermo Cat# 14301 EDTA Sigma Cat# E4884 EPPS Thermo Cat# 561476.AK Critical commercial assays CellTiter-Glo Promega Cat# G7571 iBlot2 NC ministacks Invitrogen Cat# 1323002 Caspase-Glo® 3/7 Assay System Promega Cat# G8090 Senescence β-Galactosidase Activity Assay Kit Cell Signaling Technology Cat# 23833 MycoAlert mycoplasma detection kit Lonza Biologics Cat# LT07-318 BCA protein assay kit Thermo Cat# 23227 Western blot detection kit Cytiva Cat# 45-002-401 (Continued on next page) Cell Reports Medicine 6, 102467, December 16, 2025 e2 OPEN ACCESS
Recombinant:Article Title: A triple-action PROTAC for wild-type p53 cancer therapy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies His-Tag Cell Signaling Technology Cat# 2365S; RRID: AB_2115720 FLAG-Tag Cell Signaling Technology Cat# 2368S; RRID: AB_2217020 BRD4 Cell Signaling Technology Cat# 13440S; RRID: AB_2687578 p53 (DO-1) (Pantropic) Millipore Cat# OP43-100UG; RRID: AB_10682938 p21 Waf1/Cip1 (12S1) Cell Signaling Technology Cat# 2947S; RRID: AB_823586 β-actin Sigma Cat# A1978; RRID: AB_476692 HDM2 (Ab-1) (IF2) Millipore Cat# OP46-100UG; RRID: AB_564803 HDMX/MDM4 Fortis Life Sciences Cat# A300-287A; RRID: AB_263407 Goat anti-Mouse IgG - HRP BioRad Cat# 1706516; RRID: AB_2921252 Goat anti-Rabbit IgG - HRP Cell Signaling Technology Cat# 7074; RRID: AB_2099233 Goat anti-Rabbit IgG - Poly-HRP Invitrogen Cat# 32260; RRID: AB_1965959 BRD2 (D89B4) Cell Signaling Technology Cat# 5848; RRID: AB_10835146 BRD3 (E3D5N) Cell Signaling Technology Cat# 94302 Bacterial and virus strains pET28a vector Addgene Cat# 69864-3 pET15b vector Novagen Cat# 69661 BL21(DE3) E. coli NEB Cat# C2527H pNIC28Bsa4 vector Addgene Cat# 26103 Biological samples Pediatric Leukemia Blood (Primary T-ALL) DFCI Institutional Review Board Protocol #06-078 Chemicals, peptides, and recombinant proteins Ficoll-Paque PREMIUM Fisher Scientific Cat# 45-001-751 RPMI-1640 Gibco Cat# 21780-076 EMEM Gibco Cat# 11095080 DMEM Gibco Cat# 11965118 MEM Gibco Cat# 11095098 McCoy’s 5A (Modified) Medium Gibco Cat# 16600082 Ham’s F-12K (Kaighn’s) Medium Gibco Cat# 2127022 Fetal Bovine Serum Gemini Bio Cat# 100-106 Trypan Blue Gibco Cat# 15250-076 Pen-Strep Gibco Cat# 18140-122 (+)-JQ1 MedChemExpress Cat# HY-13030 (+)-JQ1-carboxylic acid MedChemExpress Cat# HY-78695 (Continued on next page) e1 Cell Reports Medicine 6, 102467, December 16, 2025 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER RG7388 MedChemExpress Cat# HY-15676 A1874 Selleckchem Cat# S8785 dBET6 MedChemExpress Cat# HY-112588 DMSO Sigma Cat# D2650-100ML 1M HEPES, pH 7.5 Gibco Cat# 15630-080 TCEP Thermo Cat# 77720 RIPA lysis buffer Sigma Cat# 20-188 NaCl Fisher Scientific Cat# BP358-212 Human GST-HDM2 R&D Systems Cat# E3-202-050 UBE1 R&D Systems Cat# E− 305-025 UbcH5c/UBE2D3 R&D Systems Cat# E2-627-100 Mg-ATP R&D Systems Cat# B-20 FLAG-p53 BPS Bioscience Cat# 100412 6xHis-BRD4 (BD1+BD2) BPS Bioscience Cat# 31045 4-12% Bis-Tris Gels Invitrogen Cat# NP0335BOX 3-8% Tris-Acetate Gels Invitrogen Cat# EA0375BOX NuPAGE LDS Sample Buffer (4×) Thermo Cat# NP0008 Glycerol Sigma Cat# G9012-1L DTT Gold Biotechnology Cat# 27565-41-9 Isopropyl β-D-1-thiogalactopyranoside Gold Biotechnology Cat# 367-93-1 Protease inhibitor cocktail Sigma Cat# 11873580001 PhosSTOP phosphatase inhibitor Sigma Cat# 4906845001 Urea Sigma Cat# U53758 Iodoacetamide Pierce Cat# A39271 Chloroform Sigma Cat# CX1055 Methanol Fisher Scientific Cat# A412-4 LysC Wako Cat# 129-02541 Trypsin Pierce Cat# 90305 Hydroxylamine Sigma Cat# 467804 TMTpro 18-plex reagents Thermo Cat# A52045 Sep-Pal C18 Waters Cat# WAT020515 Imidazole Sigma Cat# I202 BME Thermo Cat# 21985023 IGEPAL Thermo Cat# J61055.AE PMSF Thermo Cat# 36978 Ni-NTA beads Qiagen Cat# 30210 Bortezomib Sigma Cat# 5043140001 Cycloheximide Thermo Cat# J66004.XF Dynabeads Epoxy 270 Thermo Cat# 14301 EDTA Sigma Cat# E4884 EPPS Thermo Cat# 561476.AK Critical commercial assays CellTiter-Glo Promega Cat# G7571 iBlot2 NC ministacks Invitrogen Cat# 1323002 Caspase-Glo® 3/7 Assay System Promega Cat# G8090 Senescence β-Galactosidase Activity Assay Kit Cell Signaling Technology Cat# 23833 MycoAlert mycoplasma detection kit Lonza Biologics Cat# LT07-318 BCA protein assay kit Thermo Cat# 23227 Western blot detection kit Cytiva Cat# 45-002-401 (Continued on next page) Cell Reports Medicine 6, 102467, December 16, 2025 e2 OPEN ACCESS
Modification:Article Title: A triple-action PROTAC for wild-type p53 cancer therapy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies His-Tag Cell Signaling Technology Cat# 2365S; RRID: AB_2115720 FLAG-Tag Cell Signaling Technology Cat# 2368S; RRID: AB_2217020 BRD4 Cell Signaling Technology Cat# 13440S; RRID: AB_2687578 p53 (DO-1) (Pantropic) Millipore Cat# OP43-100UG; RRID: AB_10682938 p21 Waf1/Cip1 (12S1) Cell Signaling Technology Cat# 2947S; RRID: AB_823586 β-actin Sigma Cat# A1978; RRID: AB_476692 HDM2 (Ab-1) (IF2) Millipore Cat# OP46-100UG; RRID: AB_564803 HDMX/MDM4 Fortis Life Sciences Cat# A300-287A; RRID: AB_263407 Goat anti-Mouse IgG - HRP BioRad Cat# 1706516; RRID: AB_2921252 Goat anti-Rabbit IgG - HRP Cell Signaling Technology Cat# 7074; RRID: AB_2099233 Goat anti-Rabbit IgG - Poly-HRP Invitrogen Cat# 32260; RRID: AB_1965959 BRD2 (D89B4) Cell Signaling Technology Cat# 5848; RRID: AB_10835146 BRD3 (E3D5N) Cell Signaling Technology Cat# 94302 Bacterial and virus strains pET28a vector Addgene Cat# 69864-3 pET15b vector Novagen Cat# 69661 BL21(DE3) E. coli NEB Cat# C2527H pNIC28Bsa4 vector Addgene Cat# 26103 Biological samples Pediatric Leukemia Blood (Primary T-ALL) DFCI Institutional Review Board Protocol #06-078 Chemicals, peptides, and recombinant proteins Ficoll-Paque PREMIUM Fisher Scientific Cat# 45-001-751 RPMI-1640 Gibco Cat# 21780-076 EMEM Gibco Cat# 11095080 DMEM Gibco Cat# 11965118 MEM Gibco Cat# 11095098 McCoy’s 5A (Modified) Medium Gibco Cat# 16600082 Ham’s F-12K (Kaighn’s) Medium Gibco Cat# 2127022 Fetal Bovine Serum Gemini Bio Cat# 100-106 Trypan Blue Gibco Cat# 15250-076 Pen-Strep Gibco Cat# 18140-122 (+)-JQ1 MedChemExpress Cat# HY-13030 (+)-JQ1-carboxylic acid MedChemExpress Cat# HY-78695 (Continued on next page) e1 Cell Reports Medicine 6, 102467, December 16, 2025 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER RG7388 MedChemExpress Cat# HY-15676 A1874 Selleckchem Cat# S8785 dBET6 MedChemExpress Cat# HY-112588 DMSO Sigma Cat# D2650-100ML 1M HEPES, pH 7.5 Gibco Cat# 15630-080 TCEP Thermo Cat# 77720 RIPA lysis buffer Sigma Cat# 20-188 NaCl Fisher Scientific Cat# BP358-212 Human GST-HDM2 R&D Systems Cat# E3-202-050 UBE1 R&D Systems Cat# E− 305-025 UbcH5c/UBE2D3 R&D Systems Cat# E2-627-100 Mg-ATP R&D Systems Cat# B-20 FLAG-p53 BPS Bioscience Cat# 100412 6xHis-BRD4 (BD1+BD2) BPS Bioscience Cat# 31045 4-12% Bis-Tris Gels Invitrogen Cat# NP0335BOX 3-8% Tris-Acetate Gels Invitrogen Cat# EA0375BOX NuPAGE LDS Sample Buffer (4×) Thermo Cat# NP0008 Glycerol Sigma Cat# G9012-1L DTT Gold Biotechnology Cat# 27565-41-9 Isopropyl β-D-1-thiogalactopyranoside Gold Biotechnology Cat# 367-93-1 Protease inhibitor cocktail Sigma Cat# 11873580001 PhosSTOP phosphatase inhibitor Sigma Cat# 4906845001 Urea Sigma Cat# U53758 Iodoacetamide Pierce Cat# A39271 Chloroform Sigma Cat# CX1055 Methanol Fisher Scientific Cat# A412-4 LysC Wako Cat# 129-02541 Trypsin Pierce Cat# 90305 Hydroxylamine Sigma Cat# 467804 TMTpro 18-plex reagents Thermo Cat# A52045 Sep-Pal C18 Waters Cat# WAT020515 Imidazole Sigma Cat# I202 BME Thermo Cat# 21985023 IGEPAL Thermo Cat# J61055.AE PMSF Thermo Cat# 36978 Ni-NTA beads Qiagen Cat# 30210 Bortezomib Sigma Cat# 5043140001 Cycloheximide Thermo Cat# J66004.XF Dynabeads Epoxy 270 Thermo Cat# 14301 EDTA Sigma Cat# E4884 EPPS Thermo Cat# 561476.AK Critical commercial assays CellTiter-Glo Promega Cat# G7571 iBlot2 NC ministacks Invitrogen Cat# 1323002 Caspase-Glo® 3/7 Assay System Promega Cat# G8090 Senescence β-Galactosidase Activity Assay Kit Cell Signaling Technology Cat# 23833 MycoAlert mycoplasma detection kit Lonza Biologics Cat# LT07-318 BCA protein assay kit Thermo Cat# 23227 Western blot detection kit Cytiva Cat# 45-002-401 (Continued on next page) Cell Reports Medicine 6, 102467, December 16, 2025 e2 OPEN ACCESS
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